The White Building

The White Building project was a multi-year participatory transmedia project using art as a mechanism for community building and organising in the White Building in Phnom Penh, Cambodia. The White Building in central Phnom Penh was built in 1963 as part of a modernist vision of social housing for artists and performers. Following the trauma of the Khmer Rouge, where the city was emptied and an estimated ninety percent of Cambodia’s artists were killed, the intervening Vietnamese-backed government sought to repopulate the building with an invitation to surviving artists to return. In recent years, largely due to government neglect, the building has fallen into disrepair and was demolished in June 2017. However, behind the fading facade and dilapidated infrastructure there was a complex community of over three thousand people including artists, musicians, community activists and everyday city dwellers. This research project archives and explores the role of recent media, art and creative community projects to document the everyday lives of the Building's inhabitants as both a means of resistance and to enable critical reflexivity among participants. The key question posed at the outset of development of the programs was “can localised creative art and media programs amplify the lived experience of a community and positively impact internal and external perception, identification and position?” To address this question a number of initiatives across a variety of mediums and media platforms were developed. This included development of the Aziza Film School, weekly art, photography, digital storytelling and community organising programs, events and exhibitions in the White Building showcasing creative works by residents for both internal and external audiences, partnership with local groups such as Sa Sa Art Projects who ran a gallery space and artist in residency program in the Building and fostering the development of the White Building Collective - a group of residents and students at the film school who have created high impact films, photography and online works including the Humans of Phnom Penh series. And in partnership with Sa Sa Art Projects the development of festivals, screenings and exhibitions showcasing the work of residents to the broader community and the creation of a community library and archive and the online archive of whitebuilding.org. Through this diverse range of initiatives, there was a desire to not only celebrate and document the living memory of this unique community, but to push back against government and property developers' interest in the site. The research documents how the dominant discursive acts of the more powerful can be challenged through the expression of the 'lived' and the elevation of everyday life. And that the very perception of the space and the sense of place was (re)produced through these interactions across these new and diverse mediascapes resulting in increased collective identification and action

In vitro synergy and enhanced murine brain penetration of saquinavir coadministered with mefloquine.

Highly active antiretroviral therapy has substantially improved prognosis in human immunodeficiency virus (HIV). However, the integration of proviral DNA, development of viral resistance, and lack of permeability of drugs into sanctuary sites (e.g., brain and lymphocyte) are major limitations to current regimens. Previous studies have indicated that the antimalarial drug chloroquine (CQ) has antiviral efficacy and a synergism with HIV protease inhibitors. We have screened a panel of antimalarial compounds for activity against HIV-1 in vitro. A limited efficacy was observed for CQ, mefloquine (MQ), and mepacrine (MC). However, marked synergy was observed between MQ and saquinavir (SQV), but not CQ in U937 cells. Furthermore, enhancement of the antiviral activity of SQV and four other protease inhibitors (PIs) by MQ was observed in MT4 cells, indicating a class specific rather than a drug-specific phenomenon. We demonstrate that these observations are a result of inhibition of multiple drug efflux proteins by MQ and that MQ also displaces SQV from orosomucoid in vitro. Finally, coadministration of MQ and SQV in CD-1 mice dramatically altered the tissue distribution of SQV, resulting in a >3-fold and >2-fold increase in the tissue/blood ratio for brain and testis, respectively. This pharmacological enhancement of in vitro antiviral activity of PIs by MQ now warrants further examination in vivo

Sex difference in OA : Should we blame estrogen?

Funding: The authors declared that this study has received no financial support.Peer reviewedPublisher PD

Coevolved Multidrug-Resistant HIV-1 Protease and Reverse Transcriptase Influences Integrase Drug Susceptibility and Replication Fitness

Integrase strand transfer inhibitors (InSTIs) are recommended agents in first-line combination antiretroviral therapy (cART). We examined the evolution of drug resistance mutations throughout HIV-1 pol and the effects on InSTI susceptibility and viral fitness. We performed single-genome sequencing of full-length HIV-1 pol in a highly treatment-experienced patient, and determined drug susceptibility of patient-derived HIV-1 genomes using a phenotypic assay encompassing full-length pol gene. We show the genetic linkage of multiple InSTI-resistant haplotypes containing major resistance mutations at Y143, Q148 and N155 to protease inhibitor (PI) and reverse transcriptase inhibitor (RTI) resistance mutations. Phenotypic analysis of viruses expressing patient-derived IN genes with eight different InSTI-resistant haplotypes alone or in combination with coevolved protease (PR) and RT genes exhibited similar levels of InSTI susceptibility, except for three haplotypes that showed up to 3-fold increases in InSTI susceptibility (p ≤ 0.032). The replicative fitness of most viruses expressing patient-derived IN only significantly decreased, ranging from 8% to 56% (p ≤ 0.01). Interestingly, the addition of coevolved PR + RT significantly increased the replicative fitness of some haplotypes by up to 73% (p ≤ 0.024). Coevolved PR + RT contributes to the susceptibility and viral fitness of patient-derived IN viruses. Maintaining patients on failing cART promotes the selection of fitter resistant strains, and thereby limits future therapy options

H_c_3 for a thin-film superconductor with a ferromagnetic dot

We investigate the effect of a ferromagnetic dot on a thin-film superconductor. We use a real-space method to solve the linearized Ginzburg-Landau equation in order to find the upper critical field, H_c_3. We show that H_c_3 is crucially dependent on dot composition and geometry, and may be significantly greater than H_c_2. H_c_3 is maximally enhanced when (1) the dot saturation magnetization is large, (2) the ratio of dot thickness to dot diameter is of order one, and (3) the dot thickness is large